| Solution Information | help | |
| Enzyme: | NACHT, LRR and PYD domains-containing protein 3 | |
| inhibitor: | BDBM76286 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | Assay Materials: Yeast Strain: ASC/NALP3/Caspase1 SD-Broth Growth Media: 6.8 g Yeast Nitrogen Base w/o amino acids, 8 mg arginine, 20 mg threonine, 12 mg isoleucine, 24 mg phenylalanine, 8 mg valine, supplemented with 2% alpha- D-glucose and 0.05 % leucine (5 mg/per liter). SD Broth Selection Media: 6.8 g Yeast Nitrogen Base w/o amino acids, 8 mg arginine, 20 mg threonine, 12 mg isoleucine, 24 mg phenylalanine, 8 mg valine, supplemented with 1 % galactose and 0.2% raffinose SD-media Agar: SD Broth Growth Media with 1.7 % bacto-agar. Assay Plate: Corning 1536 Well White Plate (3725) Detection Reagent: Gal-Screen ss-Galactosidase Reporter Gene Assay System for Yeast or Mammalian Cells (Applied Biosystems Group, T1030). I. Compound Addition: 1. Using a Labcyte Echo, DMSO and test compounds are transferred in to assay plates. DMSO only is transferred to columns 1-4 (Control wells), while varying volumes of test compounds are transferred to columns 4-44 to achieve the desired test concen | |
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